Biochemistry, Department of


Date of this Version



Published as Chapter 4 in Aik-Choon Tan and Paul H. Huang, eds., Kinase Signaling Networks, Methods in Molecular Biology, vol. 1636 (Springer, 2017), pp 61-70.

doi 10.1007/978-1-4939-7154-1_4,

PMID: 287304472


Copyright © 2017 Springer Science+Business Media LLC.


The ability to directly determine endogenous kinase activity in tissue homogenates provides valuable insights into signaling aberrations that underlie disease phenotypes. When activity data is collected across a panel of kinases, a unique “signaling fingerprint” is generated that allows for discrimination between diseased and normal tissue. Here we describe the use of peptide-based kinase activity sensors to fingerprint the signaling changes associated with disease states. This approach leverages the phosphorylation-sensitive sulfonamido-oxine (Sox) fluorophore to provide a direct readout of kinase enzymatic activity in unfractionated tissue homogenates from animal models or clinical samples. To demonstrate the application of this technology, we focus on a rat model of nonalcoholic fatty liver disease (NAFLD). Sox-based activity probes allow for the rapid and straightforward analysis of changes in kinase enzymatic activity associated with disease states, providing leads for further investigation using traditional biochemical approaches.