Salivary gland structure of Ctenarytaina eucalypti (MASKELL, 1890) (Hemiptera) and phloem exudate in Eucalyptus globulus LABILLARDIÈRE, 1799 (Myrtaceae)

Authors

Anamika Sharma, Charles Sturt UniversityFollow
Soundararajan Madhavan, University of Nebraska - LincolnFollow
Anantanarayanan Raman, Charles Sturt UniversityFollow
Gary S. Taylor, University of AdelaideFollow
Murray J. Fletcher, Orange Agricultural Institute & Graham Centre for Agricultural InnovationFollow

Date of this Version

2015

Citation

Polish Journal of Entomology VOL. 84: 21–32

Comments

DOI: 10.1515/pjen-2015-0003

Abstract

The structure of the salivary glands of the free-living aphalarid Ctenarytaina eucalypti, which infests multiple species of Eucalyptus in Australasia and has been introduced into many other regions of the world, is described and illustrated. The principal salivary gland is multilobed whereas the accessory gland is tubular. 1-D electrophoresis revealed proteins of approximately 58 and 64 kDa in the salivary gland extracts and proteins of similar molecular weights in the extracted plant exudates, including phloem, from infested leaves and tender shoots of E. globulus. Proteins that could fall within this range include, but are not limited to, glucosemethanol- choline-oxidoreductase (53-66 kDa), Zn-binding dehydrogenase (67 kDa) and esterase (65-96 kDa), in addition to cytochrome P-450 (50-55 kDa), trehalase (56 kDa), amylase (50-75 kDa) and lipase (48-52 kDa). Previous studies indicate that glucose-methanol-cholineoxidoreductase, Zn-binding dehydrogenase, cytochrome P-450 and trehalase suppress plantdefence mechanisms, whereas the cell-degrading enzymes such as amylase, lipase and esterase have a possible role in enabling C. eucalypti to insert its stylet into leaf and shoot tissues of E. globulus.