EXOPEPTIDASE CATALYZED SITE-SPECIFIC BONDING OF SUPPORTS, LABELS AND BIOACTIVE AGENTS TO PROTEINS

Authors

Fred W. Wagner, Walton, Nebr.
Thomas R. Coolidge, Falls Village, Conn.
Sheldon M. Schuster, Gainseville, Fla.
Jay Stout, Lincoln, Nebr.
Dwane E. Wylie, Lincoln, Nebr.
Klaus Breddam, Glostrup, Denmark
William Lewis, Lincoln, Nebr.

Date of this Version

1993

Citation

Patent Number: 5,234,820

Comments

Appl. No.: 545,915

Abstract

An auxiliary substance such as a label, support, or bioactive agent is attached to a protein at a site that is remote from the active site of the protein by the use of exopeptidase and a nucleophile which is an amino acid, amino acid derivative, amine or alcohol. In one embodiment, the nucleophile is attached to the carboxy terminus of a protein by catalysis with exopeptidase to form an adduct and then the adduct or its combination with a linker arm is bound to the auxiliary substance. In another embodiment, the auxiliary substance or its combination with a linker arm is bound to the nucleophile to form an intermediate substance which is then coupled by catalysis with exopeptidase to the carboxy terminus of a protein.