PRODUCTION OF ISOPRENE BY METHANE - PRODUCING ARCHAEA

Nicole Nicole Buan Murphy, Lincoln, NE
Karrie A. Weber, University of Nebraska-Lincoln
Jared T. Aldridge, University of Nebraska-Lincoln
Carr Robert Carr, Lincoln, NE

Document Type Article

Abstract

Plasmid vectors and use of plasmid vectors in methods for producing methane and isoprene using Archaea are dis closed. Particularly, plasmid vectors that express isoprene synthase ( ispS ) are prepared and inserted into methanogens, such as Methanosarcina acetivorans , to allow for c -production of methane and isoprene. In one embodiment, the methods of the present disclosure can be used for wastewater management.