Chlamydomonas reinhardtii selenocysteine tRNA ^[Ser]Sec

Authors

Mahadev Rao, National Institutes of Health, Bethesda, Maryland
Bradley A. Carlson, National Institutes of Health, Bethesda, MarylandFollow
Sergey V. Novoselov, University of Nebraska-Lincoln
Donald P. Weeks, University of Nebraska-LincolnFollow
Vadim Gladyshev, University of Nebraska-LincolnFollow
Dolph L. Hatfield, National Institutes of Health, Bethesda, MarylandFollow

Document Type

Article

Date of this Version

July 2003

Comments

RNA (2003), 9:923–930. Published by Cold Spring Harbor Laboratory Press. Copyright © 2003 RNA Society. Permission to use.

Abstract

Eukaryotic selenocysteine (Sec) protein insertion machinery was thought to be restricted to animals, but the occurrence of both Sec-containing proteins and the Sec insertion system was recently found in Chlamydomonas reinhardtii, a member of the plant kingdom. Herein, we used RT-PCR to determine the sequence of C. reinhardtii Sec tRNA ^[Ser]Sec, the first non-animal eukaryotic Sec tRNA ^[Ser]Sec sequence. Like its animal counterpart, it is 90 nucleotides in length, is aminoacylated with serine by seryl-tRNA synthetase, and decodes specifically UGA. Evolutionary analyses of known Sec tRNAs identify the C. reinhardtii form as the most diverged eukaryotic Sec tRNA ^[Ser]Sec and reveal a common origin for this tRNA in bacteria, archaea, and eukaryotes.