Characterization of Mammalian Selenoproteomes

Authors

• Gregory V. Kryukov, University of Nebraska-Lincoln
• Sergi Castellano, Grup de Recerca en Informàtica Biomèdica, Institut Municipal d’Investigació Mèdica, Universitat Pompeu Fabra, Centre de Regulació Genòmica
• Sergey V. Novoselov, University of Nebraska-Lincoln
• Alexey V. Lobanov, University of Nebraska-Lincoln
• Omid Zehtab, University of Nebraska-Lincoln
• Roderic Guigo, Grup de Recerca en Informàtica Biomèdica, Institut Municipal d’Investigació Mèdica, Universitat Pompeu Fabra, Centre de Regulació Genòmica, Doctor Aiguader 80, 08003 Barcelona, Catalonia, Spain
• Vadim N. Gladyshev, University of Nebraska-LincolnFollow

Document Type

Article

Date of this Version

May 2003

Comments

Published in Science, vol. 300, no. 5624 (May 30, 2003), pp. 1439– 1443. Used by permission.

Abstract

In the genetic code, UGA serves as a stop signal and a selenocysteine codon, but no computational methods for identifying its coding function are available. Consequently, most selenoprotein genes are misannotated. We identified selenoprotein genes in sequenced mammalian genomes by methods that rely on identification of selenocysteine insertion RNA structures, the coding potential of UGA codons, and the presence of cysteine-containing homologs. The human selenoproteome consists of 25 selenoproteins.