A virus-induced gene silencing method to study soybean cyst nematode parasitism in Glycine max

Authors

• Pramod K. Kandoth, University of Missouri
• Robert Heinz, University of Missouri
• Greg Yeckel, University of Missouri
• Nathan W. Gross, University of Missouri
• Parijat S. Juvale, Iowa State University
• John Hill, Iowa State University
• Steven A. Whitham, Iowa State University
• Thomas J. Baum, Iowa State University
• Melissa G. Mitchum, University of MissouriFollow

Document Type

Article

Date of this Version

2013

Citation

Kandoth et al.: A virus-induced gene silencing method to study soybean cyst nematode parasitism in Glycine max. BMC Research Notes 2013 6:255; doi:10.1186/1756-0500-6-255

Comments

Copyright 2013 Kandoth et al.; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Background: Bean pod mottle virus (BPMV) based virus-induced gene silencing (VIGS) vectors have been developed and used in soybean for the functional analysis of genes involved in disease resistance to foliar pathogens. However, BPMV-VIGS protocols for studying genes involved in disease resistance or symbiotic associations with root microbes have not been developed.

Findings: Here we describe a BPMV-VIGS protocol suitable for reverse genetic studies in soybean roots. We use this method for analyzing soybean genes involved in resistance to soybean cyst nematode (SCN). A detailed SCN screening pipeline is described.

Conclusions: The VIGS method described here provides a new tool to identify genes involved in soybean-nematode interactions. This method could be adapted to study genes associated with any root pathogenic or symbiotic associations.