Arabidopsis GLABROUS1 Gene Requires Downstream Sequences for Function

Authors

John C. Larkin, University of Nebraska-Lincoln
David G. Oppenheimer, University of Nebraska - Lincoln
Susan Pollock, University of Nebraska - Lincoln
M. David Marks, University of Nebraska - Lincoln

Document Type

Article

Date of this Version

1993

Citation

The Plant Cell, Vol. 5, 1739-1748, December 1993

Comments

Copyright 1993 American Society of Plant Physiologists

Abstract

The Arabidopsis GLABROUSl (GL1) gene is a myb gene homolog required for the initiation of trichome development. In situ hybridiration revealed that the highest levels of GL1 transcripts were present in developing trichomes. In contrast, previous work had shown that putative promoter sequences from the 5‘ noncoding region of the GL1 gene directed the expression of a β-glucuronidase (GUS) reporter gene only in stipules. Deletion analysis of the 3’ noncoding region of GL1 has identified an enhancer that is essential for GL1 function. Sequences fmm the region containing the enhancer, in conjunction with GL1 upstream sequences, direct the expression of a GUS reporter gene in leaf primordia and developing trichomes in addition to stipules, indicating that the downstream enhancer is required for the normal expression pattern of GL1.