Isolation and characterization of 11 polymorphic microsatellite loci in collared lizards (Crotaphytus collaris)

Authors

Delbert W. Hutchison, Whitman College, Walla Walla, WAFollow
Jared L. Strasburg, Washington University, St. Louis, MO
Jennifer A. Brisson, University of Nebraska-LincolnFollow
Shawn Cummings, St. Louis, MO

Date of this Version

2004

Comments

Published in Molecular Ecology Notes 4 (2004), pp. 554–556; doi: 10.1111/j.1471-8286.2004.00730.x Copyright © 2004 Blackwell Publishing Ltd. Used by permission.

Abstract

We identified 11 polymorphic microsatellite loci in collared lizards (Crotaphytus collaris). Polymorphism assessment in 512 individuals from 52 populations sampled across much of the species distribution revealed a fairly high degree of genetic diversity (six to 20 alleles per locus) and a wide range of average expected heterozygosity values (0.143–0.530). We found no evidence for linkage, very few deviations from HW expectation (two of 572 possible population/locus analyses) and thus no evidence for null alleles. There was a tendency for reduced polymorphism towards the northern periphery.