Arabidopsis G-protein interactome reveals connections to cell wall carbohydrates and morphogenesis

Authors

• Karsten Klopffleisch, University of Cologne, Cologne
• Nguyen Phan, University of North Carolina at Chapel Hill
• Kelsey Augustin, Wayne State College, Wayne
• Robert S. Bayne, University of North Carolina at Chapel Hill
• Katherine S. Booker, University of North Carolina at Chapel Hill
• Jose R. Botella, University of Queensland, Brisbane
• Nicholas C. Carpita, Purdue University, West Lafayette
• Tyrell Carr, University of North Carolina at Chapel Hill
• Jin-Gui Chen, Oak Ridge National Laboratory, Biosciences Division
• Thomas Ryan Cooke, University of Georgia, Athens
• Arwen Frick-Cheng, University of North Carolina at Chapel Hill
• Erin J. Friedman, University of North Carolina at Chapel Hill
• Brandon Fulk, University of Nebraska-Lincoln
• Michael G. Hahn, University of Georgia, Athens
• Kun Jiang, University of North Carolina at Chapel Hill
• Lucia Jorda, Universidad Polite´ cnica de Madrid
• Lydia Kruppe, University of Cologne, Cologne
• Chenggang Liu, University of North Carolina at Chapel Hill
• Justine Lorek, Max-Planck Institute for Plant Breeding Research, Cologne
• Maureen C. McCann, Purdue University, West Lafayette
• Antonio Molina, Universidad Polite´ cnica de Madrid
• Etsuko N. Moriyama, University of Georgia, AthensFollow
• M. Shahid Mukhtar, University of North Carolina at Chapel Hill & University of Alabama at Birmingham
• Yashwanti Mudgil, University of North Carolina at Chapel Hill & University of Delhi
• Sivakumar Pattathil, University of Georgia, Athens
• John Schwarz, University of North Carolina at Chapel Hill
• Steven Seta, University of North Carolina at Chapel Hill
• Matthew Tan, University of North Carolina at Chapel Hill
• Ulrike Temp, University of Cologne, Cologne
• Yuri Trusov, University of Queensland, Brisbane
• Daisuke Urano, University of North Carolina at Chapel Hill
• Bastian Welter, University of Cologne, Cologne
• Jing Yang, University of North Carolina at Chapel Hill
• Ralph Panstruga, Max-Planck Institute for Plant Breeding Research, Cologne & RWTH Aachen University, Aachen & Aachen UniversityFollow
• Joachim F. Uhrig, University of Cologne, Cologne & University of CologneFollow
• Alan M. Jones, University of North Carolina at Chapel HillFollow

Document Type

Article

Date of this Version

2011

Citation

Molecular Systems Biology 7:532 doi:10.1038/msb.2011.66

Comments

2011 EMBO and Macmillan Publishers Limited

Abstract

The heterotrimeric G-protein complex is minimally composed of Ga, Gb, and Gc subunits. In the classic scenario, the G-protein complex is the nexus in signaling from the plasma membrane, where the heterotrimeric G-protein associates with heptahelical G-protein-coupled receptors (GPCRs), to cytoplasmic target proteins called effectors. Although a number of effectors are known in metazoans and fungi, none of these are predicted to exist in their canonical forms in plants. To identify ab initio plant G-protein effectors and scaffold proteins, we screened a set of proteins from the G-protein complex using two-hybrid complementation in yeast. After deep and exhaustive interrogation, we detected 544 interactions between 434 proteins, of which 68 highly interconnected proteins form the core G-protein interactome. Within this core, over half of the interactions comprising two-thirds of the nodes were retested and validated as genuine in planta. Co-expression analysis in combination with phenotyping of loss-of-function mutations in a set of core interactome genes revealed a novel role for G-proteins in regulating cell wall modification.