PRODUCTION OF ISOPRENE BY METHANE - PRODUCING ARCHAEA

Authors

Nicole Roswitha Buan Murphy, Lincoln , NEFollow
Karrie A. Weber, Lincoln , NEFollow
Jared T. Aldridge, University of Nebraska-LincolnFollow
Robert Carr, Lincoln , NE

Document Type

Article

Date of this Version

2020

Citation

United States Patent Buan Murphy et al. Patent No.: US 10,533,192 B2

Abstract

Plasmid vectors and use of plasmid vectors in methods for producing methane and isoprene using Archaea are disclosed . Particularly, plasmid vectors that express isoprene synthase ( ispS ) are prepared and inserted into methanogens, such as Methanosarcina acetivorans, to allow for co - production of methane and isoprene. In one embodiment , the methods of the present disclosure can be used for wastewater management.