Papers in the Biological Sciences
Papers in Microbiology
Document Type
Article
Date of this Version
May 1962
Abstract
THOMPSON, P. J. (University of Nebraska, Lincoln) AND T. L. THOMPSON. Some characteristics of a purified heat-stable aldolase. J. Bacteriol. 84:694-700. 1962-Aldolase from a thermophilic strain of bacteria was obtained in a state of high purity. Heat studies of purified aldolases from cells cultivated at 45 and 65 C showed them equally stable at 70 C for 1 hr. Metal-ion and chelate studies indicated that thermal aldolase is metal ion-independent. Carboxypeptidase did not alter activity or specificity. The enzyme was specific for fructose- 1, 6-diphosphate. Hydrazine was found inhibitory in the assay procedure. The inhibition was independent of pH over the range of H+ concentrations tested and was reversed by dialysis against water.
Comments
Published in Journal of Bacteriology, volume 84, 1962. Copyright © 1962 by the American Society for Microbiology. Used by permission.