Papers in the Biological Sciences


Date of this Version

June 2005


Published in APPLIED AND ENVIRONMENTAL MICROBIOLOGY, June 2005, p. 3337–3341 Vol. 71, No. 6. Copyright © 2005, American Society for Microbiology. Used by permission.


The insecticidal protoxin from Bacillus thuringiensis has been shown to be a major component of the spore coat. We have developed a novel surface display system using B. thuringiensis spores in which the N-terminal portion of the protoxin is replaced with a heterologous protein. The expression vector with a sporulationspecific promoter was successfully used to display green fluorescent protein and a single-chain antibody (scFv) gene that encodes anti-4-ethoxymethylene-2-phenyl-2-oxazolin-5-one (anti-phOx) antibody. The spores that carry the anti-phOx antibody can bind to phOx specifically.

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