Physiology of Sporeforming Bacteria Associated with Insects: Radiorespirometric Survey of Carbohydrate Metabolism In the 12 Serotypes of Bacillus thuringiensis

Authors

Kenneth W. Nickerson, University of Nebraska-LincolnFollow
Grant St. Julian, Northern Regional Research Laboratory, Agricultural Research Service, U.S. Department of Agriculture, Peoria, Illinois
Lee A. Bulla Jr., Northern Regional Research Laboratory, Agricultural Research Service, U.S. Department of Agriculture, Peoria, Illinois

Date of this Version

July 1974

Comments

Published in APPLIED MICROBIOLOGY, JULY 1974, p. 129-132. Copyright 1974 American Society for Microbiology. Used by permission.

Abstract

Radiorespirometry was used to compare the primary pathways of glucose catabolism in 18 strains of Bacillus thuringiensis representing the 12 established serotypes. Every strain utilizes the Embden-Meyerhof-Pamas pathway almost exclusively; pentose-phosphate pathway participation is minor. The Embden- Meyerhof-Parnas pathway predominates regardless of whether the cells were grown in a minimal medium or one containing yeast extract. The results indicate that the absolute requirement for citrate and related compounds is not a result of defective citrate or glucose transport and metabolism.