Papers in the Biological Sciences


Date of this Version

February 1973


Published in JOURNAL OF BACTERIOLOGY, Feb. 1973, p. 619-626. Copyright 1973 American Society for Microbiology. Used by permission.


Two pantothenate-requiring mutants of Agrobacterium tumefaciens have been isolated. One of them (strain WMP-1) is unusual in that growth levels equivalent to the parent strain are achieved only when the medium is additionally supplemented with aspartate or another compound related to the tricarboxylic acid cycle. Extracts of cells grown on limiting aspartate were found to contain four times more 14IC-pantothenate than th6se grown at optimal aspartate concentrations. This difference was found in both the perchloric acid-soluble and -insoluble fractions, presumably the coenzyme A pool and acyl carrier protein, respectively. These findings are discussed in terms of membrane integrity and the control of fatty acid biosynthesis.

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