Agricultural and Biological Systems Engineering, Department of
Department of Agricultural and Biological Systems Engineering: Faculty Publications
Accessibility Remediation
If you are unable to use this item in its current form due to accessibility barriers, you may request remediation through our remediation request form.
Document Type
Article
Date of this Version
2-2008
Citation
OPTICS EXPRESS (3 March 2008), Vol. 16, No. 5 ;
Abstract
Abstract: We have been investigating a microfluidics platform for highspeed, low-cost sequencing of single DNA molecules using novel “chargeswitch” nucleotides. A significant challenge is the design of a flowcell suitable for manipulating bead-DNA complexes and sorting labeled polyphosphate molecules by charge. The flowcell is part of a singlemolecule detection instrument, creating fluorescence images from labeled polyphosphates. These images would ultimately be analyzed by signal processing algorithms to identify specific nucleotides in a DNA sequence. Here we describe requirements of the fluidics system for loading, identifying, tracking, and positioning beads. By dynamically modulating pressure gradients in the plenum chambers of a multi-channel network, we could guide individual beads with high precision to any desired coordinate and reversibly trap them in stepped channels. We show that DNA immobilized on pressure-trapped beads can be physically extended into a downstream channel under electric force for analysis. Custom dynamic algorithms for automated bead control are described.
Comments
©2008 Optical Society of America. Used by permission.