Resistome and mobilome in surface runoff from manured soil as affected by setback distance

Authors

Noelle Mware, University of Nebraska - LincolnFollow
Maria C. Hall, University of Nebraska-Lincoln
Selvakumar Rajendran, University of Nebraska-Lincoln
John E. Gilley, USDA-ARSFollow
Amy Schmidt, University of Nebraska-LincolnFollow
Shannon Bartelt-Hunt, University of Nebraska - LincolnFollow
Yifan Zhang, Wayne State UniversityFollow
Xu Li, University of Nebraska-LincolnFollow

Document Type

Article

Date of this Version

2022

Citation

Journal of Hazardous Materials 429 (2022) 128278

doi:10.1016/j.jhazmat.2022.128278

Comments

U.S. government work

Abstract

Land application of livestock manure introduces antibiotic resistance genes (ARGs) and mobile genetic elements (MGEs) into the soil environment. The objectives of this study were to examine the changes of resistome and mobilome in runoff and soil as a function of setback distance, i.e., the distance between manured soil and surface water, and to quantify the contributions of manure and background soil to the ARGs and MGEs in surface runoff. The resistome and mobilome in runoff and soil from a field-scale plot study were characterized using a high throughput quantitative polymerase chain reaction (HT-qPCR) array. It was estimated that a setback distance of ~40 m is required to reduce the total abundance of ARGs and MGEs in runoff from amended plots to that in control runoff. The resistome and mobilome of the soil in the setback region was not affected by manure-borne ARGs and MGEs. SourceTracker analyses revealed that background soil gradually became the predominant source of the ARGs and MGEs in runoff as setback distance increased. The results demonstrate how manure-borne ARGs and MGEs dissipated in agricultural runoff with increasing setback distance and had limited impacts on the resistome and mobilome of soil within the setback region.