Chemical and Biomolecular Engineering, Department of: Papers in Subdisciplines

 

Papers in Biotechnology

Accessibility Remediation

If you are unable to use this item in its current form due to accessibility barriers, you may request remediation through our remediation request form.

Date of this Version

June 2005

Comments

This article was Published in BioPharm International,June 2004. © 2006 Advanstar Communications. All rights reserved. Please visit BioPharm International for published version of the article.

Abstract

The introduction of new protein-based therapeutics such as monoclonal antibodies (MAbs), MAb-based vaccines, growth factors and plasma proteins implies the need to study, characterize, and purify. The separation step is likely to be a bottleneck and cost-effective technology will be needed to rectify it. The currently prevalent matrices for chromatographic separation of immunoglobulins (Igs) are based on Protein A or its recombinant versions (Protein G). They display excellent selectivity and specificity, but are expensive. A Protein A matrix costs $8,000 to 12,000 per L-resin. The typical production column volume is 100 L. The million-dollar matrix is far more expensive than the production hardware.

Share

COinS