Protocol to engineer nanofilms embedded lipid nanoparticles for controlled and targeted drug delivery (NECTAR)

Authors

Rashi Porwal, University of Nebraska-Lincoln
Stephen L. Hayward, University of Nebraska-Lincoln
Srivatsan Kidambi, University of Nebraska-Lincoln, University of Nebraska Medical CenterFollow

Date of this Version

3-17-2023

Document Type

Article

Citation

STAR Protocols 4, 101685, March 17, 2023. https://doi.org/10.1016/j.xpro.2022.101685

Comments

Open access.

Abstract

We present a protocol to engineer a substrate-mediated delivery platform comprising hyaluronic acid-coated lipid nanoparticles (HALNPs) embedded into polyelectrolyte multilayer (PEM) films. This platform allows controlled spatiotemporal release of lipid nanoparticles (LNP) by embedding them within the polyelectrolyte multilayer films matrix. HALNP conjugate with antibodies also adds the ability for targeted delivery. The use of LNP enables this platform to encapsulate both hydrophobic and hydrophilic drugs. This platform can easily be reproduced and utilized for various biomedical drug delivery applications. For complete details on the use and execution of this protocol, please refer to Hayward et al. (2015, 2016a, 2016b), Hayward and Kidambi (2018), and Kidambi and Hayward (2022).