Chemical and Biomolecular Engineering, Department of
Department of Chemical and Biomolecular Engineering: Faculty Publications
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Date of this Version
6-16-2023
Document Type
Article
Citation
STAR Protocols 4, 102158, June 16, 2023. https://doi.org/10.1016/j.xpro.2023.102158
Abstract
Numerous biology tools are developed to work for model organisms, which, however, do not work effectively in non-model organisms. Here, we present a protocol for developing a synthetic biology toolkit for Rhodopseudomonas palustris CGA009, a non-model bacterium with unique metabolic properties. We describe steps for introducing and characterizing biological devices in nonmodel bacteria, such as the utilization of fluorescence markers and RT-qPCR. This protocol may also be applicable for other non-model organisms. For complete details on the use and execution of this protocol, please refer to Immethun et al..1
Included in
Biochemical and Biomolecular Engineering Commons, Biomedical Engineering and Bioengineering Commons
Comments
Open access.