Protocol to develop a synthetic biology toolkit for the non-model bacterium R. palustris

Authors

Mark Kathol, University of Nebraska-LincolnFollow
Cheryl Immethun, University of Nebraska-Lincoln
Rajib Saha, University of Nebraska-LincolnFollow

Date of this Version

6-16-2023

Document Type

Article

Citation

STAR Protocols 4, 102158, June 16, 2023. https://doi.org/10.1016/j.xpro.2023.102158

Comments

Open access.

Abstract

Numerous biology tools are developed to work for model organisms, which, however, do not work effectively in non-model organisms. Here, we present a protocol for developing a synthetic biology toolkit for Rhodopseudomonas palustris CGA009, a non-model bacterium with unique metabolic properties. We describe steps for introducing and characterizing biological devices in nonmodel bacteria, such as the utilization of fluorescence markers and RT-qPCR. This protocol may also be applicable for other non-model organisms. For complete details on the use and execution of this protocol, please refer to Immethun et al..¹