Propofol induces ERK-dependant expression of c-Fos and Egr-1 in neuronal cells

Authors

• Srivatsan Kidambi, University of Nebraska-LincolnFollow
• Joel Yarmush, New York Methodist Hospital
• Wayne Fong, New York Methodist Hospital
• Sangeetha Kamath, New York Methodist Hospital
• Joseph SchianodiCola, New York Methodist Hospital
• Yaakov Nahmias, Massachusetts General Hospital, Shriners Hospitals for Children and Harvard Medical School

Date of this Version

2009

Document Type

Article

Citation

Neuroreport. 2009 May 6; 20(7): 657–662.

Comments

© 2009 Wolters Kluwer Health | Lippincott Williams & Wilkins. Used by permission.

Abstract

This study explored the effects of propofol on c-Fos and Egr-1 in neuroblastoma (N2A) cells. We demonstrate that propofol induced the expression of c-Fos and Egr-1 within 30 and 60 min of exposure time. At 16.8 μM concentration, propofol induced a 6 and 2.5-fold expression of c-Fos and Egr-1, respectively. However, at concentrations above 100 μM, propofol failed to induce expression of c-Fos or Egr-1. Propofol-induced c-Fos and Egr-1 transcription was unaffected by bicuculline, a γ-aminobutyric acid-A receptor antagonist, but was abolished by PD98059, a mitogen-activated protein kinase/extracellular signal-regulated kinase inhibitor. Our study shows that clinically relevant concentrations of propofol induce c-Fos and Egr-1 expression through an extracellular signalregulated kinase mediated and γ-aminobutyric acid-A independent pathway.