Propofol induces ERK-dependant expression of c-Fos and Egr-1 in neuronal cells

Authors

Srivatsan Kidambi, University of Nebraska-LincolnFollow
Joel Yarmush, New York Methodist Hospital
Wayne Fong, New York Methodist Hospital
Sangeetha Kamath, New York Methodist Hospital
Joseph SchianodiCola, New York Methodist Hospital
Yaakov Nahmias, Massachusetts General Hospital, Shriners Hospitals for Children and Harvard Medical School

Date of this Version

2009

Document Type

Article

Citation

Neuroreport. 2009 May 6; 20(7): 657–662.

Comments

© 2009 Wolters Kluwer Health | Lippincott Williams & Wilkins. Used by permission.

Abstract

This study explored the effects of propofol on c-Fos and Egr-1 in neuroblastoma (N2A) cells. We demonstrate that propofol induced the expression of c-Fos and Egr-1 within 30 and 60 min of exposure time. At 16.8 μM concentration, propofol induced a 6 and 2.5-fold expression of c-Fos and Egr-1, respectively. However, at concentrations above 100 μM, propofol failed to induce expression of c-Fos or Egr-1. Propofol-induced c-Fos and Egr-1 transcription was unaffected by bicuculline, a γ-aminobutyric acid-A receptor antagonist, but was abolished by PD98059, a mitogen-activated protein kinase/extracellular signal-regulated kinase inhibitor. Our study shows that clinically relevant concentrations of propofol induce c-Fos and Egr-1 expression through an extracellular signalregulated kinase mediated and γ-aminobutyric acid-A independent pathway.