Chemistry, Department of

 

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Document Type

Article

Date of this Version

2013

Citation

ACS Chem Biol. 2013 July 19; 8(7): 1640–1645.

Comments

Copyright © 2013 American Chemical Society

Abstract

We have utilized in vitro evolution to identify tRNA variants with significantly enhanced activity for the incorporation of unnatural amino acids into proteins in response to a quadruplet codon in both bacterial and mammalian cells. This approach will facilitate the creation of an optimized and standardized system for the genetic incorporation of unnatural amino acids using quadruplet codons, which will allow the biosynthesis of biopolymers that contain multiple unnatural building blocks.

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