‘Cassette’-ISES (In Situ Enzymatic Screening) Identifies Complementary Chiral Scaffolds for Hydrolytic Kinetic Resolution Across a Range of Epoxides

Authors

Sangeeta Dey, University of Nebraska - Lincoln
Douglas R. Powell, University of Oklahoma Norman Campus
Chunhua Hu, University of Nebraska - Lincoln
David B. Berkowitz, University of Nebraska - LincolnFollow

Citation

Angew Chem Int Ed Engl. 2007 ; 46(37): 7010–7014.

Comments

Copyright Dey et al.

doi:10.1002/anie.200701280

Abstract

‘Cassette’-ISES (In Situ Enzymatic Screening) Identifies Complementary Chiral Scaffolds for Hydrolytic Kinetic Resolution Across a Range of Epoxides

A new ‘Cassette’-In Situ Enzymatic Screen (ISES) for combinatorial catalysis is introduced. This allows the experimentalist to obtain an information-rich readout, in real time, providing an estimate of the sense and magnitude of enantioselectivity across more than one substrate. In its first iteration, the screen identified Co^III-salen catalysts with β-pinene- and α-naphthylalanine-derived chiral scaffolds with broad, yet complementary, substrate specificities.