Department of Chemistry

 

Document Type

Article

Date of this Version

2009

Citation

Patent No.: US 7,575,908 B1

Comments

Appl. No.: 11/402,298

Abstract

A method and kit for immobilization of a glycoprotein. The method may include activating an affinity Support. The affinity Support may be activated by reacting the affinity Support with a compound that is reactive with one or more functional groups included within the glycoprotein. The method may also include oxidizing the glycoprotein in which oxidation conditions are selected to yield an oxidized glycoprotein that is biologically active and contains a Sufficient number of reactive aldehyde groups for coupling to a Support. For example, the oxidized glycoprotein may include five reactive aldehyde groups. In addition, the method may include reacting the oxidized-glycoprotein with the activated affinity Sup port to immobilize the glycoprotein.

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