Characterizing RNA Dynamics at Atomic Resolution Using Solution-state NMR Spectroscopy

Authors

Jameson R. Bothe, University of Michigan
Evgenia N. Nikolova, University of Michigan
Catherine D. Eichhorn, University of MichiganFollow
Jeetender Chugh, University of Michigan
Alexandar L. Hansen, University of Toronto
Hashim M. Al-Hashimi, University of MichiganFollow

Document Type

Article

Date of this Version

2011

Citation

Nat Methods. ; 8(11): 919–931

Comments

NIH Public Access

doi:10.1038/nmeth.1735

Abstract

Many recently discovered non-coding RNAs do not fold into a single native conformation, but rather, sample many different conformations along their free energy landscape to carry out their biological function. Unprecedented insights into the RNA dynamic structure landscape are provided by solution-state NMR techniques that measure the structural, kinetic, and thermodynamic characteristics of motions spanning picosecond to second timescales at atomic resolution. From these studies a basic description of the RNA dynamic structure landscape is emerging, bringing new insights into how RNA structures change to carry out their function as well as applications in RNA-targeted drug discovery and RNA bioengineering.