Nanotoxicity of ZrS3 Probed in a Bioluminescence Test on E. coli Bacteria: The Effect of Evolving H2S

Authors

Olga Zakharova, Derzhavin Tambov State University & National University of Science and Technology “MISIS”Follow
Alexander Gusev, Derzhavin Tambov State University & National University of Science and Technology “MISIS”Follow
Jehad Abourahma, University of Nebraska—LincolnFollow
Nataliia S. Vorobeva, University of Nebraska—LincolnFollow
Dmitry V. Sokolov, National University of Science and Technology “MISIS”Follow
Dmitry S. Muratov, National University of Science and Technology “MISIS”Follow
Denis V. Kuznetsov, National University of Science and Technology “MISIS”Follow
Alexander Sinitskii, University of Nebraska-LincolnFollow

ORCID IDs

https://orcid.org/0000-0002-2027-4311

https://orcid.org/0000-0002-8688-3451

Date of this Version

2020

Citation

Nanomaterials 2020, 10, 1401; doi:10.3390/nano10071401 www.mdpi.com/journal/nanomaterials

Comments

2020 by the authors.

Abstract

Materials from a large family of transition metal trichalcogenides (TMTCs) attract considerable attention because of their potential applications in electronics, optoelectronics and energy storage, but information on their toxicity is lacking. In this study, we investigated the toxicity of ZrS3, a prominent TMTC material, toward photoluminescent E. coli bacteria in a bioluminescence test. We found that freshly prepared ZrS3 suspensions in physiological saline solution with concentrations as high as 1 g/L did not exhibit any toxic effects on the bacteria. However, ZrS3 suspensions that were stored for 24 h prior to the bioluminescence tests were very toxic to the bacteria and inhibited their emission, even at concentrations down to 0.001 g/L. We explain these observations by the aqueous hydrolysis of ZrS3, which resulted in the formation of ZrOx on the surface of ZrS3 particles and the release of toxic H2S. The formation of ZrOx was confirmed by the XPS analysis, while the characteristic H2S smell was noticeable for the 24 h suspensions. This study demonstrates that while ZrS3 appears to be intrinsically nontoxic to photoluminescent E. coli bacteria, it may exhibit high toxicity in aqueous media. The results of this study can likely be extended to other transition metal chalcogenides, as their toxicity in aqueous solutions may also increase over time due to hydrolysis and the formation of H2S. The results of this study also demonstrate that since many systems involving nanomaterials are unstable and evolve over time in various ways, their toxicity may evolve as well, which should be considered for relevant toxicity tests.