Effects of long-term fluoride exposure are associated with oxidative biochemistry impairment and global proteomic modulation, but not genotoxicity, in parotid glands of mice

Authors

Giza Hellen Nonato MirandaI, Federal University of Para
Leidiane Alencar de Oliveira Lima, Federal University of Para
Leonardo Oliveira Bittencourt, Federal University of Para
Sa´vio Monteiro dos Santos, Federal University of Para
Michel Platini Caldas de Souza, Evandro Chagas Institute
Lygia Sega Nogueira, Evandro Chagas Institute
Edivaldo Herculano Corrêa de Oliveira, Evandro Chagas Institute
Marta Chagas Monteir, Federal University of Para
Aline Dionizio, University of São Paulo
Aline Lima Leite, University of Nebraska-Lincoln
Juliano Pelim Pessan, São Paulo State University
Juliano Pelim Pessan, University of São Paulo
Marı´lia Afonso Rabelo Buzalaf, University of São Paulo
Rafael Rodrigues LimaI, Federal University of Para

Document Type

Article

Date of this Version

1-27-2022

Citation

.Miranda GHN, Alencar de Oliveira Lima L, Bittencourt LO, dos Santos SM, Platini Caldas de Souza M, Nogueira LS, et al. (2022) Effects of long-term fluoride exposure are associated with oxidative biochemistry impairment and global proteomic modulation, but not genotoxicity, in parotid glands of mice. PLoS ONE 17(1): e0261252. https://doi.org/10.1371/journal. pone.0261252

Comments

open access

Abstract

Fluoride has become widely used in dentistry because of its effectiveness in caries control. However, evidence indicates that excessive intake interferes with the metabolic processes of different tissues. Thus, this study aimed to investigate the effects of long-term exposure to F on the parotid salivary gland of mice, from the analysis of oxidative, proteomic and genotoxic parameters.

The animals received deionized water containing 0, 10 or 50 mg/L of F, as sodium fluoride, for 60 days. After, parotid glands were collected for analysis of oxidative biochemistry, global proteomic profile, genotoxicity assessment and histopathological analyses.

The results revealed that exposure to fluoride interfered in the biochemical homeostasis of the parotid gland, with increased levels of thiobarbituric acid reactive species and reduced glutathione in the exposed groups; as well as promoted alteration of the glandular proteomic profile in these groups, especially in structural proteins and proteins related to oxidative stress. However, genotoxic assessment demonstrated that exposure to fluoride did not interfere with DNA integrity in these concentrations and durations of exposure. Also, it was not observed histopathological alterations in parotid gland.