Effects of Fluoride on Submandibular Glands of Mice: Changes in Oxidative Biochemistry, Proteomic Profile, and Genotoxicity

Authors

Leidiane Alencar de Oliveira Lima, Federal University of Pará
Giza Hellen Nonato Miranda, Federal University of Pará
Walessa Alana Bragança Aragão, Federal University of Pará
Leonardo Oliveira Bittencourt, Federal University of Pará
Sávio Monteiro dos Santos, Federal University of Pará
Michel Platini Caldas de Souza, Evandro Chagas Institute
Lygia S. Nogueira, Evandro Chagas Institute
Edivaldo Herculano Corrêa de Oliveira, Evandro Chagas Institute
Marta Chagas Monteiro, Federal University of Pará
Aline Dionizio, University of São Paulo
Aline Lima Leite, University of Nebraska-Lincoln
Juliano Pelim Pessan, São Paulo State University
Marília Afonso Rabelo Buzalaf, University of São Paulo
Rafael Rodrigues Lima, Federal University of Pará

Document Type

Article

Date of this Version

9-27-2021

Citation

Lima LAdO, Miranda GHN, Aragão WAB, Bittencourt LO, dos Santos SM, de Souza MPC, Nogueira LS, de Oliveira EHC, Monteiro MC, Dionizio A, Leite AL, Pessan JP, Buzalaf MAR and Lima RR (2021) Effects of Fluoride on Submandibular Glands of Mice: Changes in Oxidative Biochemistry, Proteomic Profile, and Genotoxicity. Front. Pharmacol. 12:715394. doi: 10.3389/fphar.2021.715394

Comments

open access

Abstract

Although fluoride (F) is well-known to prevent dental caries, changes in cell processes in different tissues have been associated with its excessive exposure. Thus, this study aimed to evaluate the effects of F exposure on biochemical, proteomic, and genotoxic parameters of submandibular glands. Twenty one old rats (n = 30) were allocated into three groups: 60 days administration of drinking water containing 10 mgF/L, 50 mgF/L, or only deionized water (control). The submandibular glands were collected for oxidative biochemistry, protein expression profile, and genotoxic potential analyses. The results showed that both F concentrations increased the levels of thiobarbituric acid–reactive substances (TBARS) and reduced glutathione (GSH) and changed the proteomic profile, mainly regarding the cytoskeleton and cellular activity. Only the exposure to 50 mgF/L induced significant changes in DNA integrity. These findings reinforce the importance of continuous monitoring of F concentration in drinking water and the need for strategies to minimize F intake from other sources to obtain maximum preventive/therapeutic effects and avoid potential adverse effects.