Chemistry, Department of
Department of Chemistry: Dissertations, Theses, and Student Research
First Advisor
Lawrence J. Parkhurst
Date of this Version
Spring 4-25-2016
Document Type
Thesis
Citation
A thesis presented to the faculty of the Graduate College at the University of Nebraska in partial fulfillment of requirements for the degree of Master of Science
Major: Chemistry
Under the supervision of Professor Lawrence J. Parkhurst
Lincoln, Nebraska, April 2016
Abstract
Many of the spectrophotometric techniques used to determine the properties of intracellular human hemoglobin cannot be utilized due to the turbidity of erythrocyte suspensions. An Integrating Cavity Absorption Meter, or ICAM, allows for absorption measurements of strongly scattering samples in the visible-light region of the spectrum. The spectrum of oxygenated hemoglobin within erythrocytes is significantly different from the absorption spectrum of oxygenated hemoglobin in solution. Studies of the oxygen binding to hemoglobin in erythrocytes allowed the four sequential binding constants (Adair constants) to be determined and compared with those of hemoglobin in solution. The Adair constants for hemoglobin in solution were found to be markedly different from those of hemoglobin in the erythrocyte.
Advisor: Lawrence J. Parkhurst
Comments
Copyright © 2016, Kyle Kelly Hill. Used by permission