Catabolite Control Protein E (CcpE) Is a LysR-type Transcriptional Regulator of Tricarboxylic Acid Cycle Activity in Staphylococcus aureus

Authors

Torsten Hartmann, University of Saarland
Bo Zhang, University of Nebraska-LincolnFollow
Grégory Baronian, Université Montpellier
Bettina Schulthess, University of Zürich
Dagmar Homerova, Slovak Academy of Sciences
Stephanie Grubmuller, Technische Universitat Munchen
Erika Kutzner, Technische Universitat Munchen
Rosmarie Gaupp, University of Nebraska-Lincoln
Ralph Bertram, University of Tubingen
Robert Powers, University of Nebraska-LincolnFollow
Karen M. Eisenreich, Technische Universityt Munchen
Jan Kormanec, Slovak Academy of Sciences
Mathias Herrmann, University of Saarland
Virginie Molle, Université Montpellier
Greg A. Somerville, University of Nebraska-LincolnFollow
Markus Bischoff, University of SaarlandFollow

Document Type

Article

Date of this Version

2013

Citation

THE JOURNAL OF BIOLOGICAL CHEMISTRY VOL. 288, NO. 50, pp. 36116–36128

Comments

© 2013 by The American Society for Biochemistry and Molecular Biology, Inc.

Abstract

Background: The TCA cycle is a central metabolic pathway that facilitates the adaption of bacteria to a nutrient-limited environment.

Results: Inactivation of CcpE in Staphylococcus aureus resulted in a decreased transcription of the aconitase encoding gene citB, and reduced TCA cycle activity.

Conclusion: CcpE affects the TCA cycle via direct transcriptional control of citB.

Significance: This is the first positive regulator of TCA cycle activity identified in this pathogen.