Animal Science Department

 

Date of this Version

2011

Comments

Published in 2010 Nebraska Swine Report. Published by Extension Division, Agricultural Research Division, Institute of Agriculture and Natural Resouces, University of Nebraska-Lincoln. Copyright ©2010 Regents of the University of Nebraska.

Abstract

A preliminary experiment was conducted to evaluate the effects of crystalline lactose on the relative abundance of sodium-dependent glucose transporter (SGLT)-1 mRNA in vitro in model porcine jejunal epithelial cells (IPEC-J2). Cells were treated with low (28 mM) or high (56 mM) concentrations of lactose alone or in combination with lipopolysaccharide (LPS; 10 ng/mL). Total RNA and culture media samples from both apical and basolateral compartments were harvested at 3, 6, 12, and 24 hours following the addition of respective treatments. With respect to relative abundance of SGLT-1 mRNA, there were no interactions of lactose, LPS, and time; however, a main effect of lactose (P < 0.01) was observed. Cells treated with a high concentration of lactose had greater SGLT-1 mRNA expression compared to the control cells (P = 0.003) and low concentration lactose (P = 0.02) treated cells. With respect to glucose quantification, polarization of glucose in the basolateral rather than in the apical compartment (P = 0.04) was observed in a time-dependent manner (P < 0.001). Supplementation of lactose in LPS-treated cells reduced LPS-mediated inhibitory effect on glucose transport from the apical to basolateral compartment (P = 0.07).

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