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STUDIES OF THE ENHANCEMENT BY DIETHYLAMINOETHYL DEXTRAN OF TRANSFECTION BY PICORNAVIRUS RIBONUCLEIC ACID
Abstract
During the past 15 years, considerable study has been directed toward the demonstration of different methods for the transfection of cells by animal virus nucleic acids. Attempts have been made to deter- mine the mechanisms involved in the transfection enhancement afforded by these methods; as a first step, experiments were often directed to- ward learning whether an enhancing method or procedure was effective be- cause of the effect on the viral nucleic acid or on the host cell, or on both. About one decade ago the capacity of certain organic polycations to enhance transfection was discovered. The first experiments were with histones and protamines. Later, a derivative of a polymer of D-glucose, namely, DEAE-dextran, with a weight of about 2,000,000 daltons, was also found to be a very effective enhancer. The mechanism or mechanisms through which the polycations enhance transfection is, in large part, not clear.
Subject Area
Microbiology
Recommended Citation
MOSLIH, MOSLIH IBRAHIM, "STUDIES OF THE ENHANCEMENT BY DIETHYLAMINOETHYL DEXTRAN OF TRANSFECTION BY PICORNAVIRUS RIBONUCLEIC ACID" (1972). ETD collection for University of Nebraska-Lincoln. AAI7227413.
https://digitalcommons.unl.edu/dissertations/AAI7227413