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THE RELATIONSHIPS BETWEEN THE ROOTSTOCKS M.7, M.9 AND THE SCIONS 'GOLDEN DELICIOUS' AND 'RED DELICIOUS' OF APPLE PLANTS
Abstract
The relationships between the rootstocks and scions of apple plants were investigated by using isotopic techniques, tissue cultures and scanning electron microscope (SEM). The results of ('14)C-study suggested that RD/M.9 plants went dormant earlier and the scion RD was more compatible with M.7 rootstock than the scion GD. The result of tissue culture study revealed that calluses of GD and M.7 grew well on the same solid medium containing full strength MS salts, amino acids, 0.4 mg/l of kinetin, 2.0 mg/l of naphthaleneacetic acid (NA)), sucrose and vitamins. This could indicate that GD and M.7 calluses had many biochemical substances in common. M.9 calluses had a loose texture when grown on GD and M.7 medium. However, the M.9 calluses were compact when only NAA was reduced from 2.0 mg/l to 0.4 mg/l. RD calluses were grown successfully for tissue culture and SEM studies when cultured on modified GS and M.7 medium by decreasing MS salts to half strength and substituting sorbitol for sucrose, benzyladenine (BA) (0.2 mg/l) for kinetin and 2,4-D (2.0 mg/l) for NAA. The results showed that the GD and RD calluses grew at the same rate when grafted on M.7 calluses. Also, the growth rate of M.7 calluses was similar when cultured under GD or RD calluses. The SEM study illustrated that GD, RD and M.7 calluses had may projections on their epidermal cells, but M.9 callus cells had a smooth epidermis. Grafted calluses of GD or RD with M.7 did not separate during sectioning, but grafted calluses of GD or RD on M.9 did separate during sectioning. This might be due to the smooth surface of M.9 callus cells.
Subject Area
Botany
Recommended Citation
MOHAMMED, ABDUL-SATTAR KHAMMAS, "THE RELATIONSHIPS BETWEEN THE ROOTSTOCKS M.7, M.9 AND THE SCIONS 'GOLDEN DELICIOUS' AND 'RED DELICIOUS' OF APPLE PLANTS" (1982). ETD collection for University of Nebraska-Lincoln. AAI8228154.
https://digitalcommons.unl.edu/dissertations/AAI8228154