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THE PULSED PHOTOACOUSTIC SPECTROSCOPY OF NICOTINEAMIDE-ADENINE DINUCLEOTIDE HYDROGEN, POTASSIUM HEXACYANOCHROMATE, CHROMIUM NUCLEOTIDES, AND PYRIDOXAL PHOSPHATE (NADH, CR-ATP)
Abstract
Pulsed photoacoustic spectroscopy of nicotineamide-adenine dinucleotide, reduced form (NADH), potassium hexacyanochromate (K(,3)Cr(CN)(,6)), chromium nucleotides (i.e., Cr-ATP), and pyridoxal phosphate has been investigated. Photoacoustic spectroscopy is a potentially powerful tool for the determination of trace and ultratrace components of biochemical interest. The theory of pulsed photoacoustics is discussed along with a report on the performance of the technique in the determination of NADH. Results presented are consistent with theory, and project a lower limit of detection of 1 x 10('-8) M for NADH. A novel method of signal analysis was applied to the photoacoustic signal generated via pulsed laser excitation/transducer detection for a K(,3)Cr(CN)(,6) solution. This method was frequency analysis via a discrete Fourier transform. The frequency spectrum was found to exhibit a pronounced maximum at the transducer resonant frequency. Use of this maximum as a measure of the photoacoustic signal strength allowed for the discrimination against window absorption and various electrical and mechanical noise sources. Results of concentration vs signal intensity studies are presented and are consistent with theory. Additional biochemical systems (Cr-ATP and PLP) were examined. Chromium-ATP was investigated to obtain new information about the various chelate forms. Results show the potential of detecting Cr-ATP in trace quantities. The reaction of pyridoxal phosphate with amino acids was investigated along with the use of PAS to detect bound PLP. This study further emphasizes the potential of PAS in the detection of trace biochemical components.
Subject Area
Analytical chemistry
Recommended Citation
FISHER, MARK RICHARD, "THE PULSED PHOTOACOUSTIC SPECTROSCOPY OF NICOTINEAMIDE-ADENINE DINUCLEOTIDE HYDROGEN, POTASSIUM HEXACYANOCHROMATE, CHROMIUM NUCLEOTIDES, AND PYRIDOXAL PHOSPHATE (NADH, CR-ATP)" (1982). ETD collection for University of Nebraska-Lincoln. AAI8306480.
https://digitalcommons.unl.edu/dissertations/AAI8306480