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RELATIONSHIP OF FERROUS IRON, TOTAL IRON, AND IRON DEFICIENCY IN IRON TOLERANT AND SUSCEPTIBLE CROP PLANTS (SORGHUM, ZEA MAYS, GLYCINE MAX, PHASEOLUS)
Abstract
Extraction procedures were developed to determine ferrous iron in leaf tissues. Total and ferrous iron were determined in Sorghum bicolor (L.) Moench, Zea mays L., Glycine max L., and Phaseolus vulgaris L. genotypes that showed tolerance and susceptibility to iron deficiency. Ferrozine {3-(2-pyridyl)-5,6-bis(4-phenyl sulfonic acid)-1,2,4-triazine} was the best reagent to chelate ferrous iron, and ferrous iron could be extracted within 30 minutes from leaf tissues. Fresh samples had higher ferrous and total iron than freeze-dried or oven-dried samples. Plants grown in nutrient solutions under growth chamber conditions were placed into solutions that induced iron deficiency at predetermined growth stages. Samples were collected daily for analysis on plants that were allowed to become severely iron deficient and regreened with added iron. Total iron concentrations changed extensively and ferrous iron decreased only slightly in the chlorotic plants. Total and ferrous iron increased when plants regreened. Total iron concentrations increased in regreened plants above iron concentrations found in leaf tissue. Ferrous iron concentrations returned to those found prior to treatments. Iron deficiency symptoms were related to total iron in the new leaves, but not ferrous iron. Plants considered to be tolerant to iron deficiency had higher total iron concentrations than plants considered to be susceptible to iron deficiency. Iron tolerant plants appeared to have mechanisms to overcome iron deficiency stress that iron susceptible plants did not have. Some iron susceptible plants did not respond to added iron even after they had become severely iron deficient.
Subject Area
Botany
Recommended Citation
PIERSON, EMIL E, "RELATIONSHIP OF FERROUS IRON, TOTAL IRON, AND IRON DEFICIENCY IN IRON TOLERANT AND SUSCEPTIBLE CROP PLANTS (SORGHUM, ZEA MAYS, GLYCINE MAX, PHASEOLUS)" (1983). ETD collection for University of Nebraska-Lincoln. AAI8314911.
https://digitalcommons.unl.edu/dissertations/AAI8314911