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ORGANIZATION OF THE DSDNA GENOME OF THE CHLORELLA ALGAL VIRUS PBCV-1 (RESTRICTION MAPPING, DOUBLE-STRANDED-DNA)
Abstract
This dissertation describes the restriction site mapping of the dsDNA genome of the eukaryotic green algal virus PBCV-1 and the localization on that map of both repetitive DNA and sequences which act as origins of replication in yeast. The restriction map was generated by cloning PBCV-1 fragments into plasmid vectors and analyzing these clones for internal restriction endonuclease sites and hybridization to genomic Southern blots. The resulting restriction site map is circular and contains 336 kbp of DNA. The circularity is indicative of either a linear circularly permuted genome or a covalently closed circular genome. Evidence for two electrophoretic species of DNA is given which favors the latter organization.
Subject Area
Plant pathology
Recommended Citation
GIRTON, LOIS ELAINE, "ORGANIZATION OF THE DSDNA GENOME OF THE CHLORELLA ALGAL VIRUS PBCV-1 (RESTRICTION MAPPING, DOUBLE-STRANDED-DNA)" (1986). ETD collection for University of Nebraska-Lincoln. AAI8706232.
https://digitalcommons.unl.edu/dissertations/AAI8706232