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Further characterization of bacteriophage SN-1
Abstract
Bacteriophage SN-1 has a 62 kD protein associated with the DNA. The protein appears to be covalently linked to the DNA in that chemical agents such as high salt, detergents, reducing agents, sodium chloride, hydrochloric acid, urea, guanidine hydrochloride, hydrogen peroxide, sodium perchlorate, formamide or phenol extraction did not remove the protein from the DNA. A protein of similar size is a minor component of whole phage SN-1. Polyclonal antibodies to SN-1 DNA were produced in a Californian rabbit. The antibodies were specific for SN-1 DNA and mutants of phage SN-1, and did not react with other DNA or BSA. The insensitivity of phage SN-l indicated that the phage codes for and carries a capsid-born DNA dependant RNA polymerase. Phage SN-1 DNA is replicated between 60 and 75 minutes after infection. Upon replication the DNA reacts with antibodies against SN-1 DNA. The identity of the recognized antigenic determinant present on the DNA is unknown. Recognition may be due to the modified base present in the DNA, the DNA-associated protein, a DNA conformational structure, or to a combination of factors since the antibody is polyclonal. The anti-SN-1 DNA antibodies also recognize a 62 and 65 kD protein that is present in infected cells. The 65 kD protein appears to be processed into the 62 kD protein.
Subject Area
Microbiology|Molecular biology
Recommended Citation
Jensen, Ellen C, "Further characterization of bacteriophage SN-1" (1989). ETD collection for University of Nebraska-Lincoln. AAI8925244.
https://digitalcommons.unl.edu/dissertations/AAI8925244