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The effects of run training on natural killer cell activity and interleukin-1 alpha levels
Abstract
The purpose of this study was to determine the effect of run training on: (1) resting NK cell activity and IL-1$\alpha$ levels and (2) NK cell activity and IL-1$\alpha$ responses to submaximal exercise. Six college aged ($\bar{\rm X}$ $\pm$ SD = 21.6 $\pm$ 1.9 yrs) male volunteers, who were running less than five miles per week, participated in this investigation. Maximal oxygen consumption rate (VO$\sb2$max) was determined from an incremental treadmill test to exhaustion. Two to seven days later, the subjects completed one hours of treadmill running at a heart rate which corresponded to 70% of VO$\sb2$max with blood samples drawn prior to and immediately following the workout. The training consisted of running three times per week at a heart rate corresponding to 70% of VO$\sb2$max for seven weeks. Following the training, the maximal and submaximal treadmill tests were repeated. Blood samples were again drawn before and immediately following the one hour submaximal treadmill run. NK activity and IL-1$\alpha$ concentrations were determined for each sample time using a colorimetric tetrazolium salt, 3-(4,5-dimethyl-thiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) cytotoxic assay, and sandwich enzyme linked immunoassay (ELISA) assay, respectively. After seven weeks of training, the average treadmill speed corresponding to 70% of VO$\sb2$max and 40 minute running distance increased significantly (p $<$ 0.05). Before the training, the average NK cell activity levels were 44% pre-exercise and 58% post-exercise. Following the training, NK cell activity levels were 42 and 52% pre and post-exercise, respectively. Baseline levels (pre-exercise) of IL-1$\alpha$ were 81 pg/ml before the training and 125 pg/ml after the training. Post-exercise IL-1$\alpha$ levels were 127 and 115 pg/ml before and after training, respectively. Repeated measures ANOVA analysis showed that there were no significant main effects or interactions for either NK activity or IL-1$\alpha$ levels (p $>$ 0.05). These results suggested that baseline levels of these immune parameters and their responses to submaximal exercise were unaffected by seven weeks of run training.
Subject Area
Immunology
Recommended Citation
McDowell, Sharon L, "The effects of run training on natural killer cell activity and interleukin-1 alpha levels" (1991). ETD collection for University of Nebraska-Lincoln. AAI9133301.
https://digitalcommons.unl.edu/dissertations/AAI9133301