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Mutagenesis and RFLP applications in finger millet (Eleusine coracana (L.); Gaertner) breeding
Abstract
The objectives of the first study, on mutagenesis were to compare the effectiveness of 5 dose levels of two mutagens (EMS and X-rays) to induce variability in finger millet; and to induce male sterility and other desirable mutations. Three finger millet varieties (SDFM-63, SDFM-957 and SDFM-1072) were studied. The X-ray dose levels studied were 0 (control), 10, 20, 30, 40 and 50 Kr; while those for EMS were 0 (control), 0.2, 0.4, 0.6, 1.0 and 1.5% aqueous solutions. Based on the numbers of mutants isolated for ten traits in M$\sb2$ and confirmed by progeny testing in M$\sb3,$ the 50Kr X-ray dose was the most effective treatment for all three varieties. The 1.0% solution level was the most effective for EMS in two of the three varieties. The variety SDFM-63 was the most susceptible to both types of mutagens. Desirable mutants isolated included dwarf, long fingered, thick fingered, early maturity and male-sterile mutants. Twenty-two male-sterile mutants were isolated in M$\sb2.$ Of these, MS8 is positively male sterile, and likely controlled by a recessive nuclear gene. M$\sb4$ data is needed to confirm this postulation and to evaluate the inheritance of the other male sterile mutants. A second study, on mtDNA variation, was conducted to classify 26 ecotypes of finger millet from Africa and India into possible cytotype groups based on the Southern blot hybridization patterns obtained with three maize (Cox 1, Turf2B and 18s-5s) and one sorghum (atp 9) mitochondrial gene probes. Five restriction endonuclease enzymes were used for single digestions on total cellular DNA, for a total of 20 enzyme/probe combinations. There was generally a lack of polymorphism, with identical RFLP banding patterns in 24 of the 26 ecotypes. However, probe atp 9 hybridized to an extra, low intensity 3.6 Kb Xba 1 fragment in ecotype SDFM-1143 from Malawi; but did not hybridize to a 3.0 Kb fragment present in all other ecotypes. The Zimbabwean ecotype, SDFM-63, had an extra, low intensity 6.5 Kb Xba 1/Cox 1 band. This data enabled classification of the ecotypes into 3 cytotype groups.
Subject Area
Genetics|Molecular biology|Agronomy
Recommended Citation
Muza, Figuhr Ronald Onias, "Mutagenesis and RFLP applications in finger millet (Eleusine coracana (L.); Gaertner) breeding" (1993). ETD collection for University of Nebraska-Lincoln. AAI9415986.
https://digitalcommons.unl.edu/dissertations/AAI9415986