Off-campus UNL users: To download campus access dissertations, please use the following link to log into our proxy server with your NU ID and password. When you are done browsing please remember to return to this page and log out.
Non-UNL users: Please talk to your librarian about requesting this dissertation through interlibrary loan.
Analysis of 138 kb of DNA sequences of the Chlorella virus PBCV-1 genome
Abstract
One hundred and thirty-eight kb of DNA, including forty-five kb of DNA at the left terminus, forty-three kb of DNA at the right terminus and fifty kb of DNA located at map position 90-140 in the 330 kb Chlorella virus PBCV-1 genome were cloned, sequenced, and analyzed. Two hundred and seventy-four complete open reading frames (ORFs) larger than 195 nucleotides were identified. One hundred and forty-three of the 274 ORFs, which are densely packed on both strands of the DNA, were considered major ORFs and are likely to encode functional gene products. Thirty-eight major ORFs resemble one or more genes in the databases. The remaining 105 major ORFs are unique. Several expected genes were found in the PBCV-1 genome, including: a DNA polymerase, a large subunit of ribonucleotide diphosphate reductase, a proliferating cell nuclear antigen, protein kinases and a UV damage repair gene. These genes are probably essential for viral replication. In addition, several unexpecteded putative genes were also found, including: a fungal translation elongation factor 3 (EF-3), a nucleotide diphosphate sugar dehydrogenase, a Cu-Zn superoxide dismutase, an orthinine decarboxylase, a glycerophosphoryl diester phosphodiesterase, a D-hydroxyisocaproate dehydrogenase, a nitrilase, a restriction endonuclease and an adenine-specific DNA methyltransferase, a nonfunctional cytosine-specific DNA methyltransferase, at least three chitinases, three ORFs which could encode proteins homologous to the virus major capsid protein, and six ORFs containing ankyrin-like repeats. Some of the unexpected genes have never been found before in a virus genome. Understanding the role some of these gene products play in the viruses life cycle should provide important information on the unusual lifestyle of these viruses.
Subject Area
Molecular biology
Recommended Citation
Li, Yu, "Analysis of 138 kb of DNA sequences of the Chlorella virus PBCV-1 genome" (1994). ETD collection for University of Nebraska-Lincoln. AAI9519541.
https://digitalcommons.unl.edu/dissertations/AAI9519541