Off-campus UNL users: To download campus access dissertations, please use the following link to log into our proxy server with your NU ID and password. When you are done browsing please remember to return to this page and log out.
Non-UNL users: Please talk to your librarian about requesting this dissertation through interlibrary loan.
Genetic characterization and population genetics of stable fly (Diptera: Muscidae)
Abstract
Genetic variation in stable fly, Stomoxys calcitrans (L.) populations from Nebraska, Canada and Texas was sampled. Five of 12 allozyme loci were polymorphic (0.95 criterion). An average of 1.7 alleles per locus was observed. Observed and expected heterozygosities were 0.121 and 0.199, respectively. Nei's genetic distance between field samples averaged 0.001 (SEM = 0.001) and ranged from 0.000 to 0.005. Wright's F-statistics revealed greater variation within populations than among populations both within Nebraska and among all samples. Gene frequencies did not vary among temporal samples from a single population. PCR-RFLP analysis of 6.2 Kb of the mitochondrial DNA (mtDNA) genome with 16 restriction enzymes did not reveal variation in stable fly populations sampled from Canada, Nebraska and Texas. PCR-RFLP analysis of a 2.2 Kb fragment of the nuclear DNA ribosomal internally transcribed spacer (ITS) region revealed no variation as well. The lack of genetic differentiation among stable fly populations suggests high levels of gene flow among populations and limited local adaptation. Results suggest rapid dispersal of advantageous genes, such as insecticide resistance among populations. The low levels of variation detected by both biochemical and molecular techniques indicate a possible genetic bottleneck during the introduction of stable fly to North America. Similar results have been recorded for other species with a similar introduction and biological dispersal capability.
Subject Area
Entomology|Genetics
Recommended Citation
Szalanski, Allen L, "Genetic characterization and population genetics of stable fly (Diptera: Muscidae)" (1995). ETD collection for University of Nebraska-Lincoln. AAI9600757.
https://digitalcommons.unl.edu/dissertations/AAI9600757