Off-campus UNL users: To download campus access dissertations, please use the following link to log into our proxy server with your NU ID and password. When you are done browsing please remember to return to this page and log out.
Non-UNL users: Please talk to your librarian about requesting this dissertation through interlibrary loan.
In vitro regeneration of buffalograss (Buchloe dactyloides (Nutt.) Engelm.)
Abstract
Plant regeneration of '609', '315', NE 84-45-3 and 'Texoka' buffalograss has been achieved through dark culture of field-collected immature inflorescences. Optimal embryogenic callus (EC) induction of '609' occurred when 2,4-D at 2 to 3mg/l was added to the culture medium. Addition of BA inhibited callus growth but stimulated differentiation. No EC was produced under light condition. The male genotype NE84-45-3 exhibited significantly higher EC induction response compared to the two female genotypes. Immature inflorescences harvested in early May exhibited a much higher embryogenic response than those harvested in July and September. Medium gelled with 3 g Gelrite/l, instead of agar, favored EC induction for Texoka and '315'. Exposing calli of Texoka to 0.5, 1.0 or 2.0 mg 2,4-D/l combined with either 0.1 mg or 0.3 mg BA/l in the subculture medium initiated shoot formation, and subsequently led to the full development of somatic embryos. Gelrite in the differentiation medium also favored somatic embryogenesis for Texoka. Addition of AgNO$\sb3$ (5-20mg/l) significantly enhanced EC production of male immature inflorescence culture of NE84-45-3 and Texoka. This effect, however, is genotype and environment dependent. No stimulatory effect of AgNO$\sb3$ was observed on EC induction for female immature inflorescence culture. Calli initiated on AgNO$\sb3$-containing media had more shoot-regenerating calli than those initiated on AgNO$\sb3$-free media. BA at 0.5 mg/l gave the best response for regeneration of NE84-45-3, regardless of the callus source. Shoot regeneration of a seeded buffalograss 'Cody' has been achieved through mature caryopsis culture. BA in the culture medium reduced callus production but significantly increased the shoot-producing callus formation. Addition of 2,4-D at 6mg/l is optimal for callus induction and shoot production. Dark treatment significantly reduced callus production. All dark treatments except a brief 1-week exposure significantly reduced shoot-producing callus formation. Embryogenic callus was obtained from leaf base and seedling segment cultures of 6-day to 12-day old seedlings. Regardless of the explant types, no EC was obtained from 3-day old seedlings.
Subject Area
Plant propagation
Recommended Citation
Fei, Shui-Zhang, "In vitro regeneration of buffalograss (Buchloe dactyloides (Nutt.) Engelm.)" (1997). ETD collection for University of Nebraska-Lincoln. AAI9819693.
https://digitalcommons.unl.edu/dissertations/AAI9819693