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Utilization of AFLP's in the introgression of the Bt transgene into an elite maize line
Abstract
Simulations have shown the utilization of molecular markers to be a potentially powerful tool in the recovery of the recurrent parent in backcross projects. The objective of this investigation was to compare the use of phenotypic selection to a combination of AFLP marker assisted selection and phenotypic selection in a maize backcross project. The project goal was the recovery of the recurrent parent genome using backcrossing after initial transfer of a Bt transgene from a donor line. Selection methods were applied to each of four maize subsets, in four generations (BC2, BC3, BC3S1, BC3S2). Analysis of data, both combined across generations and within the BC3S2 generation, resulted in similar conclusions. First, positive phenotypic selection was found to be effective used per se in recovery of the recurrent parent. Secondly, no gain was realized using AFLP markers exclusively over phenotypic selection as the initial selection criterion in the BC1 generation. Finally, there was no gain in the recovery rate of the recurrent parent utilizing AFLP marker assisted selection combined with phenotypic selection over exclusive phenotypic selection after four generations of application. Sub-optimal lab and field conditions were identified that when rectified might increase the efficiency of using AFLP markers.
Subject Area
Agronomy|Genetics
Recommended Citation
Meyer, David Herbert, "Utilization of AFLP's in the introgression of the Bt transgene into an elite maize line" (1998). ETD collection for University of Nebraska-Lincoln. AAI9829527.
https://digitalcommons.unl.edu/dissertations/AAI9829527