Graduate Studies

 

First Advisor

Shannon Bartelt-Hunt

Date of this Version

4-2024

Document Type

Article

Citation

A thesis presented to the faculty of the Graduate College at the University of Nebraska in partial fulfillment of requirements for the degree of Master of Science

Major: Civil Engineering

Under the supervision of Professor Shannon Bartelt-Hunt

Lincoln, Nebraska, April 2024

Comments

Copyright 2024, Moriah J. Brown. Used by permission

Abstract

Runoff from agricultural fields poses a significant threat to water bodies, carrying sediments, nutrients, and chemicals, including antibiotics and antibiotic-resistant bacteria (ARBs), which degrade water quality. Despite their crucial role in disease prevention and growth promotion in agriculture, antibiotics' presence in watersheds raises environmental and health concerns, particularly regarding the spread of antibiotic resistance. This study focuses on the Shell Creek watershed in east-central Nebraska, an agriculturally dominated area, where antibiotics are extensively used in farming practices. Bi-weekly grab samples are collected from four locations along Shell Creek and are processed for bacteria and antibiotics. Results indicated infrequent antibiotic detections, mostly below quantification limits, while ionophores, notably monensin, were more frequently detected. The presence of antibiotic-resistant bacteria highlights the potential risks associated with agricultural practices and antibiotic use in the watershed, highlighting the need for sustainable management strategies to mitigate water contamination and safeguard public health and ecosystems.

Advisor: Shannon Bartelt-Hunt

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