Imaging the Oocysts in the Midgut of the Mosquito

Authors

• Hillary Guzik, University of Nebraska-Lincoln

Date of this Version

2016

Document Type

Project

Citation

University of Nebraska-Lincoln Department of Entomology Online Masters Program Final Project. Lincoln, Nebraska.

Comments

Copyright 2016 Hillary Guzik

Abstract

● Learn how to dissect the midgut from the mosquito ○ Protocol on how to conduct the dissection ● Produce a portfolio of high end microscopy images of the oocysts inside of the midgut ○ Using Leica SP5 Point Scanning Confocal (Analytical Imaging Facility at Einstein College of Medicine) ○ Reconstruct the oocyst in 3D ○ To further be used in Dr. Kami Kim’s research ● Develop an image analysis protocol to automatically count each individual oocyst ○ Using Volocity analysis software (PerkinElmer) ○ Gather total counts and area measurements of individual oocysts ○ Written for future use ○ To further be used in Dr. Kami Kim’s research