Effects of SmartStax^® and SmartStax^® PRO maize on western corn rootworm (Diabrotica virgifera virgifera LeConte) larval feeding injury and adult life history parameters

Authors

Jordan D. Reinders, University of Nebraska - Lincoln
William J. Moar, CropScience Division
Graham P. Head, CropScience Division
Safeer Hassan, CropScience Division
Lance J. Meinke, University of Nebraska - LincolnFollow

Document Type

Article

Date of this Version

7-10-2023

Citation

Reinders JD, Moar WJ, Head GP, Hassan S, Meinke LJ (2023) Effects of SmartStax® and SmartStax® PRO maize on western corn rootworm (Diabrotica virgifera virgifera LeConte) larval feeding injury and adult life history parameters. PLoS ONE 18(7): e0288372. https://doi.org/ 10.1371/journal.pone.0288372

Comments

Open access.

Abstract

Field-evolved resistance of the western corn rootworm (WCR), Diabrotica virgifera virgifera LeConte, to Bacillus thuringiensis Berliner (Bt) proteins Cry3Bb1 and Cry34/35Ab1 (now classified as Gpp34Ab1/Tpp35Ab1) expressed in the pyramid SmartStax^® has been documented in areas of the United States (U.S.) Corn Belt. SmartStax^® PRO is a recently registered rootworm-active pyramid containing the same Bt proteins expressed in SmartStax^® plus DvSnf7 dsRNA. Little to no published data is available comparing efficacy of the technologies or potential effects of dietary exposure on adult WCR fitness. Therefore, experiments were conducted to compare effects of adult WCR dietary exposure to SmartStax^® and SmartStax^® PRO on life history parameters and efficacy of the technologies in the field with both Bt-susceptible and Bt-resistant WCR populations. WCR life history parameters evaluated included adult longevity, head capsule width, egg production, and egg viability. Results of small-plot field trials indicated that both technologies provided a high level of root protection when a Bt-susceptible WCR population was present. Root protection was reduced on SmartStax^® but maintained on SmartStax^® PRO when WCR Bt resistance occurred. Lifetime egg production was the key life history parameter that was significantly reduced when either Bt-susceptible or Bt-resistant adult WCR were fed SmartStax^® or SmartStax^® PRO diet. A potential fitness advantage was apparent as egg production was significantly higher in the Bt-resistant than Bt-susceptible population. The similar response by the Bt-susceptible WCR population to SmartStax^® and SmartStax^® PRO indicates that results were caused by sublethal dietary exposure to Bt proteins. Adult size (males < females) and egg viability (high: >95%) were not significantly different among treatments but longevity results were inconsistent between years. Collectively, the field efficacy and life history parameter data expand existing knowledge of SmartStax^® and SmartStax^® PRO technologies, which will inform practical WCR resistance management programs.