Evidence of Field-Evolved Resistance to Bifenthrin in Western Corn Rootworm (Diabrotica virgifera virgifera LeConte) Populations in Western Nebraska and Kansas

Authors

Adriano E. Pereira, University of Nebraska-LincolnFollow
Haichuan Wang, University of Nebraska-LincolnFollow
Sarah N. Zukoff, Kansas State UniversityFollow
Lance Meinke, University of Nebraska--LincolnFollow
B. Wade French, USDA-ARS, Brookings, SDFollow
Blair Siegfried, University of Nebraska--LincolnFollow

Document Type

Article

Date of this Version

11-13-2015

Citation

Pereira AE, Wang H, Zukoff SN, Meinke LJ, French BW, Siegfried BD (2015) Evidence of Field- Evolved Resistance to Bifenthrin in Western Corn Rootworm (Diabrotica virgifera virgifera LeConte) Populations in Western Nebraska and Kansas. PLoS ONE 10(11): e0142299. doi:10.1371/journal. pone.0142299

Comments

Public domain (US government work)

Abstract

Pyrethroid insecticides have been used to control larvae or adults of the western corn rootworm (WCR), Diabrotica virgifera virgifera LeConte, a key pest of field corn in the United States. In response to reports of reduced efficacy of pyrethroids in WCR management programs in southwestern areas of Nebraska and Kansas the present research was designed to establish a baseline of susceptibility to the pyrethroid insecticide, bifenthrin, using susceptible laboratory populations and to compare this baseline with susceptibility of field populations. Concentration-response bioassays were performed to estimate the baseline susceptibility. From the baseline data, a diagnostic concentration (LC99) was determined and used to test adults of both laboratory and field populations. Larval susceptibility was also tested using both laboratory and field populations. Significant differences were recorded in adult and larval susceptibility among WCR field and laboratory populations. The highest LC50 for WCR adults was observed in populations from Keith 2 and Chase Counties, NE, with LC50s of 2.2 and 1.38 μg/vial, respectively, and Finney County 1, KS, with 1.43 μg/vial, as compared to a laboratory non-diapause population (0.24 μg/vial). For larvae, significant differences between WCR field and laboratory populations were also recorded. Significant differences in mortalities at the diagnostic bifenthrin concentration (LC99) were observed among WCR adult populations with western Corn Belt populations exhibiting lower susceptibility to bifenthrin, especially in southwestern Nebraska and southwestern Kansas. This study provides evidence that resistance to bifenthrin is evolving in field populations that have been exposed for multiple years to pyrethroid insecticides. Implications to sustainable rootworm management are discussed.