Molecular characterization of western corn rootworm pyrethroid resistance

Authors

Dariane Souza, University of Nebraska-LincolnFollow
Blair D. Siegfried, University of FloridaFollow
Lance Meinke, University of Nebraska-LincolnFollow
Nicholas J. Miller, University of Nebraska-LincolnFollow

ORCID IDs

Dariane Souza https://orcid.org/0000-0003-3681-9053

Date of this Version

10-3-2021

Citation

Published in Pest Managment Science 77 (2021), 860–868.

DOI:10.1002/ps.6090

Comments

Copyright © 2020 Society of Chemical Industry. Published by Wiley. Used by perrmission.

Abstract

Background: Western corn rootworm (WCR) pyrethroid resistance has been confirmed in the western US Corn Belt. Toxicological and biochemical studies indicated that multiple mechanisms of resistance might be involved in the resistance trait, such as enhanced metabolism and/or kdr target-site mutation(s) in the voltage-gated sodium channels. To characterize the mechanisms of WCR pyrethroid resistance at the molecular level, pairwise comparisons were made between RNA-Seq data collected from pyrethroid-resistant and -susceptible WCR populations. Gene expression levels and sodium channel sequences were evaluated.

Results: Seven transcripts exhibited significantly different expression (q ≤ 0.05) when comparing field-collected pyrethroidresistant (R-Field) and -susceptible (S-Field) WCR populations. Three of the differentially expressed transcripts were P450s overexpressed in R-Field (9.2–26.2-fold). A higher number (99) of differentially expressed transcripts was found when comparing laboratory-derived pyrethroid-resistant (R-Lab) and -susceptible (S-Lab) WCR populations. Eight of the significant transcripts were P450s overexpressed in R-Lab (2.7–39.8-fold). This study did not detect kdr mutations in pyrethroid-resistant WCR populations. Other differentially expressed transcripts that may play a role in WCR pyrethroid resistance are discussed.

Conclusion: This study revealed that P450-mediated metabolism is likely to be a major mechanism of WCR pyrethroid resistance, which could affect the efficacy of other insecticides sharing similar metabolic pathways. Additionally, results suggested that although laboratory selection of a pyrethroid-resistant WCR population may help to characterize resistance mechanisms, a field-selected population provided rare and perhaps major variants corresponding to the resistance trait.