Distinct gene expression profiles between human preterm-derived and adult-derived intestinal organoids exposed to Enterococcus faecalis: a pilot study

Authors

• Andrea C. Masi, Newcastle University
• Tatiana Y. Fofanova, Baylor College of Medicine
• Christopher A. Lamb, Newcastle University, Newcastle upon Tyne Hospitals NHS Foundation Trust
• Jennifer Auchtung, University of Nebraska - LincolnFollow
• Robert A. Britton, Baylor College of Medicine
• Mary K. Estes, Baylor College of Medicine
• Sasirekha Ramani, Baylor College of Medicine
• Simon J. Cockell, Newcastle University
• Jonathan Coxhead, Newcastle University
• Nicholas D. Embleton, Newcastle Upon Tyne Hospitals NHS Trust, Newcastle University
• Janet E. Berrington, Newcastle Upon Tyne Hospitals NHS Trust
• Joseph F. Petrosino, Baylor College of Medicine, The Baylor College of Medicine
• Christopher J. Stewart, Newcastle University

Date of this Version

6-17-2023

Document Type

Article

Citation

Published online 2021 Dec 17. doi: 10.1136/gutjnl-2021-326552

Comments

HHS Public Access

Abstract

We read with interest the study by Kayisoglu et al¹ comparing the gene expression between embryo-derived and adult-derived intestinal organoids. Some innate immune system genes were differentially expressed between the two organoid types, suggesting a potential role of exposure to the environment, including gut microbiota, in shaping the intestinal gene expression. In extremely preterm infants (<32 weeks gestation), microbial–host interaction at the epithelial surface has been associated with various morbidities including late onset sepsis and necrotising enterocolitis.² Thus, preterm intestinal organoids may provide a specific and robust model for this population.