Distinct gene expression profiles between human preterm-derived and adult-derived intestinal organoids exposed to Enterococcus faecalis: a pilot study

Authors

Andrea C. Masi, Newcastle University
Tatiana Y. Fofanova, Baylor College of Medicine
Christopher A. Lamb, Newcastle University, Newcastle upon Tyne Hospitals NHS Foundation Trust
Jennifer Auchtung, University of Nebraska - LincolnFollow
Robert A. Britton, Baylor College of Medicine
Mary K. Estes, Baylor College of Medicine
Sasirekha Ramani, Baylor College of Medicine
Simon J. Cockell, Newcastle University
Jonathan Coxhead, Newcastle University
Nicholas D. Embleton, Newcastle Upon Tyne Hospitals NHS Trust, Newcastle University
Janet E. Berrington, Newcastle Upon Tyne Hospitals NHS Trust
Joseph F. Petrosino, Baylor College of Medicine, The Baylor College of Medicine
Christopher J. Stewart, Newcastle University

Date of this Version

6-17-2023

Document Type

Article

Citation

Published online 2021 Dec 17. doi: 10.1136/gutjnl-2021-326552

Comments

HHS Public Access

Abstract

We read with interest the study by Kayisoglu et al¹ comparing the gene expression between embryo-derived and adult-derived intestinal organoids. Some innate immune system genes were differentially expressed between the two organoid types, suggesting a potential role of exposure to the environment, including gut microbiota, in shaping the intestinal gene expression. In extremely preterm infants (<32 weeks gestation), microbial–host interaction at the epithelial surface has been associated with various morbidities including late onset sepsis and necrotising enterocolitis.² Thus, preterm intestinal organoids may provide a specific and robust model for this population.