Food Science and Technology Department


Department of Food Science and Technology: Faculty Publications

Document Type


Date of this Version



BAOJ Food Sci&Tec 2: 12


© 2018 Sapna Chitlapilly Dass, et al.

Open access


Effective cleaning strategies in a food processing facility play an important role in reducing food borne illness or economic losses associated with spoilage. In this study, the efficacy of cleaning practices was assessed by studying the bacterial population present before and after sanitation in a fluid milk processing environment. The bacterial population was studied using 16S rRNA amplicon metagenomics sequencing of the V1-V3 regions. Relative abundance of varying levels(0.01-99%)were found in the various processing facility environmental samples. Genera such as Paenibacillus, Viribacillus and Lysinibacillus that can cause food spoilage in cold-stored products were found on product contact surfaces before and after sanitation. Listeria and Yersenia were found on personal protective equipment but were not found on product contact surfaces. There were overlapping bacterial communities between various zones of the processing facility. Zones that were in contact with product had significant overlapping bacterial communities (ANOVA p<0.05) and the zones that were in close proximity to the product contact surface within the processing environment. While there were no overlaps between product contact surfaces and zones that were outside the processing area. Results from this study provide an overview on the efficacy of the cleaning process in the fluid milk processing facility, and how next generation sequencing can be utilized to improve sanitation.

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